Nanogenerators from Electrical Discharge

Electrical discharge is generally considered as a negative effect in the electronic industry and often causes electrostatic discharge (ESD) and thus failure of electronic components and integrated circuits (IC). However, this effect was recently used to develop a new energy-harvesting technology, direct-current triboelectric nanogenerator (DC-TENG). In this chapter, its fundamental mechanism and the working modes of the nanogenerator will be presented. They are different from the general alternating current TENG (AC-TENG) invented in 2012, which is based on triboelectrification and electrostatic induction. Taking advantage of the electrostatic discharge, it can not only promote the miniaturization trend of TENG and self-powered systems, but also provide a paradigm shifting technique to in situ gain electrical energy

Small-Scale Energy Harvesting from Environment by Triboelectric Nanogenerators

The increasing needs to power trillions of sensors and devices for the Internet of Things require effective technology to harvest small-scale energy from renewable natural resources. As a new energy technology, triboelectric nanogenerators (TENGs) can harvest ambient mechanical energy and convert it into electricity for powering small electronic devices continuously. In this chapter, the fundamental working mechanism and fundamental modes of a TENG will be presented. It can harvest all kinds of mechanical energy, especially at low frequencies, such as human motion, walking, vibration, mechanical triggering, rotating tire, wind, moving automobile, flowing water, rain drops, ocean waves, and so on. Such variety of energy harvesting methods promises TENG as a new approach for small-scale energy harvesting

Methyl eucomate

The crystal structure of the title compound [systematic name: methyl 3-carboxy-3-hydr­oxy-3-(4-hydroxy­benz­yl)propanoate], C12H14O6, is stabilized by inter­molecular O—H⋯O and C—H⋯O hydrogen bonds. The mol­ecules are arranged in layers, parallel to (001), which are inter­connected by the O—H⋯O hydrogen bonds

Flavonoid Ampelopsin Inhibits the Growth and Metastasis of Prostate Cancer In Vitro and in Mice

The objective of this study was to evaluate the chemopreventive effect of a novel flavonoid, ampelopsin (AMP) on the growth and metastasis of prostate cancer cells. AMP showed the more potent activity in inhibiting the proliferation of androgen-sensitive LNCaP and, to less extent, androgen-independent PC-3 human prostate cancer cell lines in vitro, primarily by induction of apoptosis associated with down-regulation of bcl-2. On the other hand, AMP showed much less activity in inhibiting the proliferation of normal prostate epithelial cells than that of prostate cancer cell lines. AMP also inhibited the migration and invasion of PC-3 cells in vitro associated with down-regulation of CXCR4 expression. In the animal study using an orthotopic prostate tumor model, AMP (150 and 300 mg/kg body weight) inhibited the growth of PC-3 tumors and lymph node and lung metastases in a dose-dependent manner. Compared to the control mice, mice treated with AMP at 300 mg/kg BW had reduced final tumor weight by 49.2% (P<0.05), lymph node metastases by 54.5% (P = 0.3) and lung metastases by 93% (P<0.05), but had no apparent alteration on food intake or body weight. The in vivo anti-growth and anti-metastasis activities of AMP were associated with induction of apoptosis and inhibition of proliferation of prostate cancer cells, reduction of prostate tumor angiogenesis, and reduction of CXCR4 expression. Our results provide supporting evidence to warrant further investigation to develop AMP as a novel efficacious and safe candidate agent against progression and metastasis of prostate cancer

Ion-Exchange Chromatography Coupled With Dynamic Coating Capillary Electrophoresis for Simultaneous Determination of Tropomyosin and Arginine Kinase in Shellfish

Tropomyosin (TM) and arginine kinase (AK) are known as two major allergens in seafood. For the first time, we demonstrate a newly developed ion-exchange chromatography coupled with dynamic coating capillary electrophoresis (IEC-DCCE) method to simultaneously analyze the TM and AK in shellfish. First, we have optimized the procedure of IEC for simple enrichment of TM and AK crude extract. By using 30 mM borate-borax at pH 9.0 with 0.3% (v/v) Tween-20 as a dynamic coating modifier for capillary electrophoresis (CE) separation, the migration time, separation efficiency and electrophoretic resolution greatly improved. The limits of detection (LOD) were 1.2 μg mL−1 for AK and 1.1 μg mL−1 for TM (S/N = 3), and the limits of quantification (LOQ) were 4.0 μg mL−1 for AK and 3.7 μg mL−1 for TM (S/N = 10). The recovery of AK ranged from 91.5 to 106.1%, while that of TM ranged from 94.0 to 109.5%. We also found that only when the concentrations of AK and TM were above LOD reported here, these proteins can stimulate human mast cell (LAD2) degranulation. Finally, the use of IEC-DCCE to analyze fresh shellfish samples highlights the applicability of this method for the simultaneous detection of these allergens in complex food systems

Characterization of the Small RNA Transcriptomes of Androgen Dependent and Independent Prostate Cancer Cell Line by Deep Sequencing

Given the important roles of miRNA in post-transcriptional regulation and its implications for cancer, characterization of miRNA facilitates us to uncover molecular mechanisms underlying the progression of androgen-independent prostate cancer (PCa). The emergence of next-generation sequencing technologies has dramatically changed the speed of all aspects of sequencing in a rapid and cost-effective fashion, which can permit an unbiased, quantitive and in-depth investigation of small RNA transcriptome. In this study, we used high-throughput Illumina sequencing to comprehensively represent the full complement of individual small RNA and to characterize miRNA expression profiles in both the androgen dependent and independent Pca cell line. At least 83 miRNAs are significantly differentially expressed, of which 41 are up-regulated and 42 are down-regulated, indicating these miRNAs may be involved in the transition of LNCaP to an androgen-independent phenotype. In addition, we have identified 43 novel miRNAs from the androgen dependent and independent PCa library and 3 of them are specific to the androgen-independent PCa. Function annotation of target genes indicated that most of these differentially expressed miRNAs tend to target genes involved in signal transduction and cell communication, epically the MAPK signaling pathway. The small RNA transcriptomes obtained in this study provide considerable insights into a better understanding of the expression and function of small RNAs in the development of androgen-independent prostate cancer

A Novel Strategy to Reconstruct NDVI Time-Series with High Temporal Resolution from MODIS Multi-Temporal Composite Products

Vegetation indices (VIs) data derived from satellite imageries play a vital role in land surface vegetation and dynamic monitoring. Due to the excessive noises (e.g., cloud cover, atmospheric contamination) in daily VI data, temporal compositing methods are commonly used to produce composite data to minimize the negative influence of noise over a given compositing time interval. However, VI time series with high temporal resolution were preferred by many applications such as vegetation phenology and land change detections. This study presents a novel strategy named DAVIR-MUTCOP (DAily Vegetation Index Reconstruction based on MUlti-Temporal COmposite Products) method for normalized difference vegetation index (NDVI) time-series reconstruction with high temporal resolution. The core of the DAVIR-MUTCOP method is a combination of the advantages of both original daily and temporally composite products, and selecting more daily observations with high quality through the temporal variation of temporally corrected composite data. The DAVIR-MUTCOP method was applied to reconstruct high-quality NDVI time-series using MODIS multi-temporal products in two study areas in the continental United States (CONUS), i.e., three field experimental sites near Mead, Nebraska from 2001 to 2012 and forty-six AmeriFlux sites evenly distributed across CONUS from 2006 to 2010. In these two study areas, the DAVIR-MUTCOP method was also compared to several commonly used methods, i.e., the Harmonic Analysis of Time- Series (HANTS) method using original daily observations, Savitzky–Golay (SG) filtering using daily observations with cloud mask products as auxiliary data, and SG filtering using temporally corrected composite data. The results showed that the DAVIR-MUTCOP method significantly improved the temporal resolution of the reconstructed NDVI time series. It performed the best in reconstructing NDVI time-series across time and space (coefficient of determination (R2 = 0.93 ~ 0.94) between reconstructed NDVI and ground-observed LAI). DAVIR-MUTCOP method presented the highest robustness and accuracy with the change of the filtering parameter (R2 = 0.99 ~ 1.00, bias = 0.001, root mean square error (RMSE) = 0.020). Only MODIS data were used in this study; nevertheless, the DAVIR-MUTCOP method proposed a universal and potential way to reconstruct daily time series of other VIs or from other operational sensors, e.g., AVHRR and VIIRS

MicroRNA-206: Effective Inhibition of Gastric Cancer Progression through the c-Met Pathway

MicroRNAs are endogenous short chain nucleotide RNAs that regulate gene function by direct binding of target mRNAs. In this study, we investigated the effects of microRNA-206 (miR-206) on the development of gastric cancer. miR-206 was first confirmed to be downregulated in gastric cancer specimens. Conversely, upregulation of c-Met was confirmed in tissue samples of human gastric cancer, with its level inversely correlated with miR-206 expression. Introduction of miR-206 inhibited cellular proliferation by inducing G1 cell cycle arrest, as well as migration and invasion. Moreover, important proliferation and/or migration related molecules such as c-Met, CDK4, p-Rb, p-Akt and p-ERK were confirmed to be downregulated by Western blot analysis. Targeting of c-Met also directly affected AGS cell proliferation, migration and invasion. In vivo, miR-206 expressing tumor cells also displayed growth delay in comparison to unaffected tumor cells. Our results demonstrated that miR-206 suppressed c-Met expression in gastric cancer and could function as a potent tumor suppressor in c-Met overexpressing tumors. Inhibition of miR-206 function could contribute to aberrant cell proliferation and migration, leading to gastric cancer development

Targeted Deletion of the Murine Lgr4 Gene Decreases Lens Epithelial Cell Resistance to Oxidative Stress and Induces Age-Related Cataract Formation

Oxidative stress contributes to the formation of cataracts. The leucine rich repeat containing G protein-coupled receptor 4 (LGR4, also known as GPR48), is important in many developmental processes. Since deletion of Lgr4 has previously been shown to lead to cataract formation in mice, we sought to determine the specific role that Lgr4 plays in the formation of cataracts. Initially, the lens opacities of Lgr4−/− mice at different ages without ocular anterior segment dysgenesis (ASD) were evaluated with slit-lamp biomicroscopy. Lenses from both Lgr4−/− and wild-type mice were subjected to oxidation induced protein denaturation to assess the ability of the lens to withstand oxidation. The expression of antioxidant enzymes was evaluated with real-time quantitative PCR. Phenotypically, Lgr4−/− mice showed earlier onset of lens opacification and higher incidence of cataract formation compared with wild-type mice of similar age. In addition, Lgr4−/− mice demonstrated increased sensitivity to environmental oxidative damage, as evidenced by altered protein expression. Real-time quantitative PCR showed that two prominent antioxidant defense enzymes, catalase (CAT) and superoxidase dismutase-1 (SOD1), were significantly decreased in the lens epithelial cells of Lgr4−/− mice. Our results suggest that the deletion of Lgr4 can lead to premature cataract formation, as well as progressive deterioration with aging. Oxidative stress and altered expression of several antioxidant defense enzymes contribute to the formation of cataracts

Characterization of Human Coronavirus Etiology in Chinese Adults with Acute Upper Respiratory Tract Infection by Real-Time RT-PCR Assays

BACKGROUND: In addition to SARS associated coronaviruses, 4 non-SARS related human coronaviruses (HCoVs) are recognized as common respiratory pathogens. The etiology and clinical impact of HCoVs in Chinese adults with acute upper respiratory tract infection (URTI) needs to be characterized systematically by molecular detection with excellent sensitivity. METHODOLOGY/PRINCIPAL FINDINGS: In this study, we detected 4 non-SARS related HCoV species by real-time RT-PCR in 981 nasopharyngeal swabs collected from March 2009 to February 2011. All specimens were also tested for the presence of other common respiratory viruses and newly identified viruses, human metapneumovirus (hMPV) and human bocavirus (HBoV). 157 of the 981 (16.0%) nasopharyngeal swabs were positive for HCoVs. The species detected were 229E (96 cases, 9.8%), OC43 (42 cases, 4.3%), HKU1 (16 cases, 1.6%) and NL63 (11 cases, 1.1%). HCoV-229E was circulated in 21 of the 24 months of surveillance. The detection rates for both OC43 and NL63 were showed significantly year-to-year variation between 2009/10 and 2010/11, respectively (P<0.001 and P = 0.003), and there was a higher detection frequency of HKU1 in patients aged over 60 years (P = 0.03). 48 of 157(30.57%) HCoV positive patients were co-infected. Undifferentiated human rhinoviruses and influenza (Flu) A were the most common viruses detected (more than 35%) in HCoV co-infections. Respiratory syncytial virus (RSV), human parainfluenza virus (PIV) and HBoV were detected in very low rate (less than 1%) among adult patients with URTI. CONCLUSIONS/SIGNIFICANCE: All 4 non-SARS-associated HCoVs were more frequently detected by real-time RT-PCR assay in adults with URTI in Beijing and HCoV-229E led to the most prevalent infection. Our study also suggested that all non-SARS-associated HCoVs contribute significantly to URTI in adult patients in China